It is the aim of this central
project to provide highly sensitive analytical techniques suitable
for studying biological molecules. So far, the following repertoire
of methods has been established:
2D-Gelelectrophoresis, electroblotting in conjunction with protein
staining, N-terminal sequencing or Western-blotting, computer-based
comparison of protein patterns after 2D-gelelectrophoresis, in
situ digestion of proteins after gelelectrophoresis and subsequent
identification by mass fingerprints of resulting peptides using
MALDI-TOF-MS and MALDI-TOF-MS/MS.
Analysis of glycolipids, oligosaccharides, glycopeptides and
glycoproteins by on-line nano-LC-ESI-MS/MS and/or off-line nano-ESI-MS/MS,
MALDI-TOF-MS, methylation analysis and multi-dimensional HPLC-fractionation.
Listed techniques may be used in the following studies:
- Identification and sequencing of proteins,
- structural studies on glycoproteins and glycolipids,
- localization of posttranslational modifications (e. g., glycosylation,
phosphorylation, phosphocholine-substitution and sulfation)
as well as
- analysis of protein/protein-interactions in protein complexes.
Dr. Hildegard Geyer
Dr. Monica Linder
Dr. Sigrid Schmitt