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Invasion and Replication Strategies of Pathogenes
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SP Z1

Structural Analysis of Biomolecules


Prof. Dr. Rudolf Geyer

Dr. Günter Lochnit
Biochemisches Institut (FB 11), Justus-Liebig-Universität, Gießen



Summary

It is the aim of this central project to provide highly sensitive analytical techniques suitable for studying biological molecules. So far, the following repertoire of methods has been established:

2D-Gelelectrophoresis, electroblotting in conjunction with protein staining, N-terminal sequencing or Western-blotting, computer-based comparison of protein patterns after 2D-gelelectrophoresis, in situ digestion of proteins after gelelectrophoresis and subsequent identification by mass fingerprints of resulting peptides using MALDI-TOF-MS and MALDI-TOF-MS/MS.

Analysis of glycolipids, oligosaccharides, glycopeptides and glycoproteins by on-line nano-LC-ESI-MS/MS and/or off-line nano-ESI-MS/MS, MALDI-TOF-MS, methylation analysis and multi-dimensional HPLC-fractionation.

Listed techniques may be used in the following studies:
  1. Identification and sequencing of proteins,
  2. structural studies on glycoproteins and glycolipids,
  3. localization of posttranslational modifications (e. g., glycosylation, phosphorylation, phosphocholine-substitution and sulfation) as well as
  4. analysis of protein/protein-interactions in protein complexes.

Involved scientists:

Dr. Hildegard Geyer
Dr. Monica Linder
Dr. Sigrid Schmitt

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