Sub-Projects of SFB 535
Invasion and Replication Strategies of Pathogenes
<< back  
SFB Homepage Organisation / Contact Members Announcements
Research Program Sub-Projects Publications Current
Workbench Public Seminars Internal Seminars Search
SP Z4

Cryoelektronmicroscopy


Dr. Martin Hardt
Central Biotechnology Unit , Justus-Liebig-Universität, Gießen


Summary

The aim of the central project Z4 is to provide modern electron microscopic methods for studying the structure of viruses and other pathogens with a focus on cryoelectron microscopy. In contrast to conventional approaches it offers the following advantages:

- the structure of pathogens can be studied in a native state avoiding the artefacts of chemical
  fixation, contrasting and drying,

- stages of infection can be snap shot by cryofixation and differentiated with high resolution in the
  time domain,

- using the appropriate equipment and preparative conditions, structural information can be
  resolved down to the nanometer or angstroem scale.

The investigation of pathogens in a native state however requires to consider the aspects of biohazards and can be done only in a laboratory providing sufficient biological safety (at least containment level 2).

So far a new high resolution transmission electron microscope was partly funded by the German Research Foundation and is now installed. The EM-lab is completely rebuilt according to the requirements of containment level 2 allowing research on GMO including the risk group 3**.

Further aims of this project are:

- to set up and to adapt the equipment and the methods for cryofixation and cryoelectron    microscopy,

- to further develop Cryo-EM methods with respect to the approved concept of biological safety    using model organisms, e.g. woodchuck hepatitis virus,

-  to establish methods of computer based image analysis including 3D modelling,

-  to complement the Cryo-EM approach using modern immune-EM methods,

-  to study the native state macromolecular structures, of the surface structures of pathogens and
   of early stages of infection in collaboration with the other subprojects of the CRC.

Cooperations with the following subprojects are planed: A2, A3, A12, A13, B1, B4, B5, B8, B9, B10, B12, B13, B14

>> Publications
 
SFB Homepage Organisation / Contact Members Announcements
Research Program Sub-Projects Publications Current
Workbench Public Seminars Internal Seminars Search
<< back | ^ Top of Page